We narrowed to 10,569 results for: fus
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Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-pEGFP-C1
Plasmid#166762PurposeTo increase the length of the linker between Venus and Noxa proteinsDepositorAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]
Plasmid#125204PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low controlDepositorInsertmCerulean3-spacer-cpVenus[E172]
UseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-TIR1-NOSt
Plasmid#108545PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-GFP
Plasmid#107709PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterEF1alphaAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationA4VPromoterMET17prAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/UAA-mEGFP-IRES-mCherry
Plasmid#49234PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/CFP.Hygro
Plasmid#46861Purposeencodes Flag-tagged mouse IL-17RA fused to Cyan Fl. ProteinDepositorInsertIL-17RA-CFP
TagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
cBEST2
Plasmid#234658PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterkasO*17tss and kasOp*Available SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16-3W
Plasmid#234424PurposeExpress APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linkerDepositorInsertAPT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-LAMP1
Plasmid#222318PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG
Plasmid#217740PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL2 (ADGRL2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-830Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
Plasmid#217708PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL3 (ADGRL3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-841Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG
Plasmid#217739PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-844Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG
Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only