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pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1954

Plasmid

#195689

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A/L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2707

Plasmid

#195636

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2753

Plasmid

#195637

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2754

Plasmid

#195638

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2755

Plasmid

#195639

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2756

Plasmid

#195640

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2757

Plasmid

#195641

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Mef2c-puro

Plasmid

#198228

Purpose

Retroviral expression of Mef2c

Depositor

Li Qian

Article

Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

Insert

Mef2c (Mef2c Mouse)

Use

Retroviral

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA.3-V5-SPOP (R221C)

Plasmid

#197022

Purpose

Mammalian expression vecor for V5-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R221C

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-eGFP-SAPAP3

Plasmid

#176853

Purpose

Expresses SAPAP3 protein fused with eGFP at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

eGFP-SAPAP3

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-eGFP-SAPAP3

Plasmid

#176859

Purpose

Expresses SAPAP3 protein fused with eGFP at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

eGFP-SAPAP3

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ1 ear

Plasmid

#199177

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ1 ear domain (Ap1g1 Mouse)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ2 ear

Plasmid

#199178

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ2 ear domain (AP1G2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA.3-V5-SPOP (R354H)

Plasmid

#197023

Purpose

Mammalian expression vecor for V5 tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R354H

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197958

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-Bam_13-36_AG

Plasmid

#148830

Purpose

Bacterial Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-V186E_AG

Plasmid

#148818

Purpose

Insect Expression of DmCaf40-V186E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-V186E (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvHN-DmBam_13-36_AF

Plasmid

#148742

Purpose

Bacterial Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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