We narrowed to 51,082 results for: Eng

1. pAC149-pCR8-dCas9VP160

   Plasmid

   #48221

   Purpose

   dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP160

   Mutation

   D10A;H840A nuclease-deficient

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

   Plasmid

   #89887

   Purpose

   Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-HRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX2-STIM1

   Plasmid

   #89817

   Purpose

   Express HUMAN STIM1

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Retroviral

   Tags

   NO TAG

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

   Plasmid

   #89886

   Purpose

   Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-NRasQ61K
   

   Use

   Unspecified

   Tags

   mCherry

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOP2A sgRNA1

   Plasmid

   #138190

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOP2A sgRNA2

   Plasmid

   #138191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-HA-Akt1-aa1-408

   Plasmid

   #73412

   Purpose

   Expresses HA tagged aa1-408 of AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 1-408 of AKT1

   Promoter

   CMV

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

    Plasmid

    #99144

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-pro-siRNA-V2-TP53

    Plasmid

    #111089

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    TP53 (TP53 Human)
    

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119067

    Purpose

    Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSI-check2-CUL1-3'UTR

    Plasmid

    #97156

    Purpose

    For dual luciferase report assay

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    CUL1 3'UTR (CUL1 Human)
    

    Expression

    Mammalian

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-Itprip-V5

    Plasmid

    #120241

    Purpose

    Expresses V5-tagged Itprip in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Itprip (ITPRIP Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2/GD-IRES-GFP-CTNNB1

    Plasmid

    #192868

    Purpose

    Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

    Depositor

    David Largaespada

    Article

    Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

    Inserts

    luc+
    EGFP
    CTNNB1
    

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

    Plasmid

    #119065

    Purpose

    Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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