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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,592 results for: eng

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Showing: 8561 - 8580 of 14592 results

  1. BE3-hA3A-Y130F

    Plasmid
    #132945
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-hA3A(Y130F)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(Y130F)
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132945-plasmid-map-sequence-id-314826

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  3. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  4. pcDNA3-His:FRB:nls:VPR

    Plasmid
    #135987
    Purpose
    Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB domain fused to the VPR activation domain
    Tags
    His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135987-plasmid-map-sequence-id-268598

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  10. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

    Plasmid
    #118807
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Publication
    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TARDBP mutant (TARDBP Human)
    Expression
    Mammalian
    Mutation
    Deletion of NTD in full-length TDP43
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    118807-plasmid-map-sequence-id-229887

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  17. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

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  18. pUC19-E3CY56

    Plasmid
    #103075
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103075-plasmid-map-sequence-id-201714

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  20. sgALK(Chr. Inv.)

    Plasmid
    #85569
    Purpose
    Expresses sgRNA targeting human ALK between exon 20 and 21
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    anaplastic lymphoma receptor tyrosine kinase (ALK Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 8561 - 8580 of 14592 results