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Plasmid#79488PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoterDepositorInsertEF1 Promoter (EEF1A1 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterEF1 PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 Stop
Plasmid#84895PurposeDONOR vector for Gateway cloning of TAF15DepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆UBR
Plasmid#81063PurposeGateway entry vector encoding full length UBR5 W1235L (∆UBR)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
UseCloningTagsExpressionMutationW1235L (bp g3704t)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆HECT
Plasmid#81065PurposeGateway entry vector encoding full length UBR5 C2768A (∆HECT)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
UseCloningTagsExpressionMutationC2768A (bp t8302g and g8303c)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 No Stop
Plasmid#84888PurposeDONOR vector for Gateway cloning of EWSR1 No StopDepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationno stopPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1-no stop
Plasmid#67345PurposehSar1 without stop codon in Gateway Entry vectorDepositorInsertSAR1 (SAR1A Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARA-no stop
Plasmid#67347PurposehSara without stop codon in Gateway Entry vectorDepositorInsertSARA (SAR1B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IL6.Pro
Plasmid#79491PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoterDepositorInsertIl6 Promoter (IL6 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterIL6 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-EWSR1 Stop
Plasmid#84887PurposeDONOR vector for Gateway cloning of EWSR1DepositorInsertEWSR1 (EWSR1 Human)
UseGateway donorTagsExpressionMutationPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
R777-E221 Hs.RALGDS
Plasmid#70505PurposeGateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)DepositorInsertRALGDS (RALGDS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 E179K
Plasmid#60541PurposeDonor vector for human MLK1 E179K mutantDepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationE179KPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1 D294A
Plasmid#60542PurposeDonor vector for human MLK1 D294A mutantDepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationD294APromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
HK2 gRNA (BRDN0001162212)
Plasmid#76310Purpose3rd generation lentiviral gRNA plasmid targeting human HK2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
221-AID-RPB1
Plasmid#195108PurposeTargeting vector to introduce AID tag in N-terminal of the human RPB1 gene. Auxin-inducible degron system.DepositorInsertAID
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p221z-dCAS9p-t35s
Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK7 gRNA (BRDN0001162216)
Plasmid#76077Purpose3rd generation lentiviral gRNA plasmid targeting human CDK7DepositorInsertCDK7 (CDK7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-MICU1-mEF
Plasmid#31765DepositorInsertMICU1 (MICU1 Human)
UsePdonr gatewayTagsNoneExpressionMutationMutated in both EF-hand domains: D231A, E242K, D4…PromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Reb_221 aka PAS628
Plasmid#71233PurposeReb_1 with RebA(G90A)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebA(G53A, G90A)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221MBPc-AID-T7RNAP
Plasmid#167975PurposeExpresses fusion protein MBPc-AID-T7RNAP in E. coliDepositorInsertMBPc-AID-T7RNAP
UseTagsMBPc and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆PABC
Plasmid#81064PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
UseCloningTagsExpressionMutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a72…PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-POLD3
Plasmid#183197PurposeEntry cloneDepositorInsertCas9-POLD3
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
UseTagsT7RNAP and TrxAExpressionBacterialMutationPromoterPtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Human, Synthetic)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterCCL5 PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC5
Plasmid#183200PurposeEntry cloneDepositorInsertCas9-RFC5
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 No Stop
Plasmid#84896PurposeDONOR vector for Gateway cloning of TAF15 No StopDepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationNo StopPromoternoneAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pABM-D4-miR30-L1221
Plasmid#132933PurposeLentivector for luciferase (control) knockdown and blasticidin resistanceDepositorInsertluciferase shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterSFFVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A66V
Plasmid#166817PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationA66VPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 - myc ULK1 wt
Plasmid#27623DepositorInsertmouse myc ULK1 (Myc Mouse)
UseGateway donr vectorTagsmycExpressionMutationInitial M from ULK1 removed. Silent mutation at A…PromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 S1213A
Plasmid#166824PurposeGateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationS1213APromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TrpV1 scFv [N221/17]
Plasmid#182082PurposeMammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.DepositorInsertrecombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)
UseTagsSortase, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 P1214R
Plasmid#166822PurposeGateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationP1214RPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPU-huTRIMmiR30-L1221
Plasmid#132937PurposeAll-in-one huTRIM5 rescue-control shRNA knockdownDepositorInserthuman TRIM5
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-HMGB1
Plasmid#183198PurposeEntry cloneDepositorInsertCas9-HMGB1
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TrpV1 scFv [N221/17]
Plasmid#190540PurposeMammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.DepositorInsertTrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC4
Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP.HuR.S221D
Plasmid#110351PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationS221D, P237SPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARFRP1-no stop
Plasmid#67343PurposehArfrp1 without stop codon in Gateway Entry vectorDepositorInsertARFRP1 (ARFRP1 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
XE221 hWnt-16-pCS2P+
Plasmid#16843DepositorInserthWnt-16 (WNT16 Human)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 M77T
Plasmid#166818PurposeGateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationM77TPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A54T
Plasmid#166815PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationA54TPromoterAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-Val15AlafsX11
Plasmid#24732DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationdeleted amino acids 44-62. deletion/frameshift…PromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
PRKCI gRNA (BRDN0001162218)
Plasmid#76403Purpose3rd generation lentiviral gRNA plasmid targeting human PRKCIDepositorInsertPRKCI (PRKCI Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS cFgfr2 (CT#221)
Plasmid#14003DepositorInsertFgfr2 (FGFR2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K651R
Plasmid#84528PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK651RPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL8B-no stop
Plasmid#67335PurposehArl8b without stop codon in Gateway Entry vectorDepositorInsertARL8B (ARL8B Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL6-no stop
Plasmid#67329PurposehArl6 without stop codon in Gateway Entry vectorDepositorInsertARL6 (ARL6 Human)
UseGateway entry vectorTagsExpressionBacterialMutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only