We narrowed to 19,403 results for: 109

1. pET28b-NmCas9-Flag D16A

   Plasmid

   #109031

   Purpose

   D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NK609

   Plasmid

   #109078

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   nanoluc
   

   Use

   Choanoflagellate expression

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. NK588

   Plasmid

   #109077

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   pActAvrII_nanoluc
   

   Use

   Choanoflagellate expression

   Mutation

   Eliminate NcoI site in the promoter

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. NK606

   Plasmid

   #109076

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   nanoluc
   

   Use

   Choanoflagellate expression

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NK587

   Plasmid

   #109075

   Purpose

   Reporter gene expression in S. rosetta

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   nanoluc
   

   Use

   Choanoflagellate expression

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. NK583

   Plasmid

   #109072

   Purpose

   Parental vector for making S. rosetta expression vectors

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   PTSG_10057
   

   Use

   Choanoflagellate expression

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NK600

   Plasmid

   #109068

   Purpose

   Parental vector for making S. rosetta expression vectors

   Depositor

   Nicole King

   Article

   Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

   Insert

   PTSG_02256
   

   Use

   Choanoflagellate expression

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415-IMP2'

   Plasmid

   #10992

   Depositor

   Francisco Malagon

   Insert

   IMP2' (IMP2' Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   Wild type

   Available Since

   Dec. 29, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJCNM(del22-68)

   Plasmid

   #1095

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acids 20 - 68 DELETED from NM domain of SUP…

   Available Since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXR002: EF1a-dCasRx-2A-EGFP

    Plasmid

    #109050

    Purpose

    Catalytically inactive dCasRx with 2A-EGFP

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-X1-Neo-GFP-FAM134B

    Plasmid

    #109026

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    EIF-1a promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEM705 Gsz

    Plasmid

    #109355

    Purpose

    GsX chimera with Gαz C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsz chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-CtIP

    Plasmid

    #109403

    Purpose

    Expresses Cas9 fusion to human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    CtIP (RBBP8 Human)
    

    Mutation

    full length CtIP

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-HE

    Plasmid

    #109400

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
    

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEM705 Gsq

    Plasmid

    #109356

    Purpose

    GsX chimera with Gαq C-terminus

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsq chimera
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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