-
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 wt
Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS_129
Plasmid#160652PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC13A5_STOP
Plasmid#161111PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC13A5 (SLC13A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D
Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
V5-ERG-R367K
Plasmid#118624PurposeExpresses V5 tagged ERG R367K (DNA binding mutant) proteinDepositorInsertERG (R367K DNA binding mutant) (ERG Human)
UseLentiviralTagsV5ExpressionMammalianMutationR367KPromoterEF1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(1-180)-2A-mCherry-puro
Plasmid#231976PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosisDepositorInsertMLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX MLKL(S345D)-2A-mCherry-puro
Plasmid#231975PurposeTet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosisDepositorInsertMLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)
UseLentiviralTagsExpressionMutationMLKL S345DPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-DmrB-Casp8-2A-mCherry-puro
Plasmid#231971PurposeTet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosisDepositorInsertCasp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-EWS_RRM(359-447)
Plasmid#188045PurposeExpresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.DepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis8ExpressionBacterialMutationdeleted amino acids 1-358, 446-656PromoterT7Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-EWS_RRM-RGG(359-513)
Plasmid#188046PurposeExpresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.DepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis8ExpressionBacterialMutationdeleted amino acids 1-358, 514-656PromoterT7Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEmal10H-MBP-EWSR1-449-513
Plasmid#199439PurposeExpresses RG2 (the second RG-rich region, 449-513) of EWSDepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis10 and MBPExpressionBacterialMutationdeleted amino acids 1-448, 514-656PromoterT7Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro
Plasmid#231973PurposeTet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosisDepositorInsertRipk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_NbH11-D4
Plasmid#155363PurposeExpresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertNb H11-D4
UseTagsHis6ExpressionMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLZRS-ER-Rac1-Q61L-shortlinker
Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.PromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), Y160A (EcI) MetNIQ in pBAD
Plasmid#118260PurposeN295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala) …PromoterNone and araBAD PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ρ1-EM GABAA receptor in pEZT-BM
Plasmid#213072PurposeExpress human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICDDepositorInsertHuman GABAa rho1 receptor (GABRR1 Human)
UseBacmam, baculovirus, oocyte expressionTagsTwin-Strep tag and superfolderGFPExpressionMammalianMutationEncodes residues S58–L383 and D451–S479, separate…PromoterCMV and T7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only