We narrowed to 2,727 results for: hal.2

1. pAAV-hSyn1-2xNLS-mTurquoise2

   Plasmid

   #118025

   Purpose

   An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Promoter

   hSyn1

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-2xNLS-mTurquoise2

   Plasmid

   #104053

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Promoter

   GFAP

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-eYFP-f

   Plasmid

   #104057

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP-f
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VV201: ChR2(C128S)-eGFP in fubi

   Plasmid

   #58510

   Purpose

   Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   ChR2(C128S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Cysteine 128 to Serine

   Promoter

   Ubiquitin

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP315

   Plasmid

   #134994

   Purpose

   KRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pUBC-KRAB-NLS-DpnI(aa146-254)
   

   Tags

   DpnI(aa146-254) and KRAB domain

   Expression

   Mammalian

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15 PemrR-gfp biosensor

   Plasmid

   #185793

   Purpose

   The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

   Depositor

   Steven Hallam

   Article

   Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

   Insert

   pEmrR-gfp
   

   Tags

   EGFP

   Expression

   Bacterial

   Promoter

   PemrR

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-eNme2-T.1-ABE8e

   Plasmid

   #185668

   Purpose

   Expresses eNme2-T.1-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNUT N6His Y95F/Y188F hTFNG

   Plasmid

   #69601

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

   Insert

   mutated human serum transferrin (TF Human)
   

   Tags

   Hexa His tag and N-terminal signal peptide, 4 aa …

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

   Promoter

   SV40

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-CRY2-CreN

    Plasmid

    #26888

    Purpose

    Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2-CreN (cre, CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    aa 19-104 of Cre

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRPML2-YFP

    Plasmid

    #18830

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRPML2-HA

    Plasmid

    #18828

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

13. TRPML2-MYC

    Plasmid

    #18829

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. TRPML2-CFP

    Plasmid

    #18831

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3-mitoT

    Plasmid

    #174519

    Purpose

    Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

    Depositor

    Oleh Khalimonchuk

    Article

    Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

    Insert

    MitoT synthetic tether
    

    Tags

    6xHis and eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV and cmv

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Cry2 mCherry GRK3 ct in pcDNA3.1

    Plasmid

    #64213

    Purpose

    mCherry fused between Cry2 and GRK3 ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK3 ct (GRK3 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAC-DELTA

    Plasmid

    #53273

    Purpose

    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    Trc

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-myc-Miro2 Δ593-618

    Plasmid

    #47901

    Purpose

    Expresses myc tagged Miro2 lacking transmembrane domain

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 Δ593-618 (RHOT2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Δ593-618, lacks transmembrane domain. Also see De…

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGG-PIP12

    Plasmid

    #196750

    Purpose

    Contains FLS2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    FLAGELLIN-SENSITIVE 2 (FLS2 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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