We narrowed to 66,338 results for: nin

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Flag-HA-USP20

Plasmid

#22573

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP20 (USP20 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMXs-c-Myc-IP

Plasmid

#15921

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Publication

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

Plasmid

#107941

Purpose

Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R)

Tags

3x HA and NLS (nucleoplasmin)

Expression

Mammalian

Mutation

E174R, S542R and K548R

Promoter

CAG

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA)

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAGMKOSiE

Plasmid

#20865

Depositor

Keisuke Kaji

Publication

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)

Expression

Mammalian

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJ241-FLEX

Plasmid

#18925

Purpose

FLEX switch empty backbone

Depositor

Scott Sternson

Publication

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

FLEX switch

Use

Cre/Lox; Subcloning

Mutation

An internal Spe1 site is used for subcloning a ge…

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCRZeroT

Plasmid

#120276

Purpose

Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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TetOn-mCherry-eGFP-RAMP4

Plasmid

#109014

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pNAHA

Plasmid

#44169

Depositor

John Olsen

Publication

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

hemagglutinin gene
neuraminidase gene

Expression

Mammalian

Mutation

T55A

Promoter

CMV enhancer/human Ferritin light chain promoter …

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTDH3-dCas9-Mxi1

Plasmid

#46921

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-Mxi1

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Publication

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-T7-SpRY-P2A-EGFP (RTW4830)

Plasmid

#139989

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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FLAG-Foxo1ADA(pCMV5)

Plasmid

#12149

Depositor

Domenico Accili

Publication

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

Threonine 24 to Alanine (A) and Serine 253 to Asp…

Promoter

CMV

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-HA-OTUD5

Plasmid

#22610

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUD5 (OTUD5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV/TO

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLEX_306

Plasmid

#41391

Purpose

Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

Depositor

David Root

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5

Expression

Mammalian

Promoter

PGK

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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