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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]

Plasmid

#226766

Purpose

Rab35(S22N)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35-mutant S22N (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]

Plasmid

#226765

Purpose

Rab35(Q67L)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35-mutant Q67L (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0216 CMVTO-hIL6Ra_M4-rens-hIL6-rens-IL6Rb

Plasmid

#231194

Purpose

Multi-cageIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6Ra_M4-rens-hIL6-rens-IL6Rb (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

Plasmid

#231208

Purpose

Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)

Use

Lentiviral

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0251 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens

Plasmid

#231216

Purpose

myc-CleaveIL-10

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

c-myc-mono-hIL10 (R5A11M)-rens (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

Plasmid

#231205

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0091 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra

Plasmid

#231191

Purpose

CageIL-10-monomer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10-R5A11M-rens-3GS-IL10Ra (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved

Plasmid

#231215

Purpose

Cleaved-myc-IL-10

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

c-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA173 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc

Plasmid

#231214

Purpose

CageIL-10-myc

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 CI

Plasmid

#227191

Purpose

Inducible lentiviral expression of CI (Catalytically Inactive) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

H309N

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-CGBE1

Plasmid

#198557

Purpose

Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-CGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234-TetO-AtoBpegRNA

Plasmid

#226668

Purpose

MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

hU6-TetO-AtoBpegRNA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-TetO

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3346 PB-nicking guide-blast

Plasmid

#226665

Purpose

PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

protective nicking sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-IRAK4-mScarlet-P2A-Puro

Plasmid

#221237

Purpose

Stable expression of mouse IRAK4-mScarlet after Lentiviral transduction

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

IRAK4 (Irak4 Mouse)

Use

Lentiviral

Tags

mScarlet

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NKp46-Fc

Plasmid

#221857

Purpose

Express human NKp46 (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

NKp46 (NCR1 Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL475

Plasmid

#219503

Purpose

Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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