We narrowed to 14,592 results for: eng

Showing: 8581 - 8600 of 14592 results

Results per page:

Sort results by:

NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FHp5pUP95C3,5SGW (E5)

Plasmid

#74035

Purpose

lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Publication

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya-Farnesyl-5

Plasmid

#56643

Purpose

Localization: Membrane, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Farnesyl (HRAS Human)

Tags

mPapaya

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
VV228: sdChR(C138S E154A)-TS in fck

Plasmid

#58523

Purpose

Expresses sdChR(C138S E154A) in mammalian cells

Depositor

Adam Cohen

Publication

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S, E154A)-TS

Use

Lentiviral

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine; changed Glutamate…

Promoter

a-CamKII

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a13-69

Plasmid

#49815

Purpose

expression vector for SRAP(13-69) in pTWIN1 vector

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-69 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
hSRAP.TWIN1.a13-87

Plasmid

#49816

Purpose

expression plasmid for SRAP(13-87) in pTWIN1 vector

Depositor

Thomas Cech

Publication

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-87 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Publication

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Publication

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-Cx35-M1-FLAG-T2A-mCherry

Plasmid

#255638

Purpose

Encodes floxed (DIO) M. americana Cx35, codon optimized for mouse expression and c-terminally fused FLAG tag, T2A self-cleaving peptide and mCherry. Mutation of K221E. hSyn promoter.

Depositor

Kafui Dzirasa

Publication

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx35

Use

AAV

Tags

FLAG-T2A-mCherry

Mutation

K221E

Promoter

hSyn

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Publication

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0007-PB-4XnrUAS-H2B-BFP

Plasmid

#236121

Purpose

Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream

Depositor

Xiaojing Gao

Publication

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B (H2BC11 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

E1b minimal promoter with four Gal4-binding sites…

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Publication

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Publication

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Expression

Mammalian

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CROPseq-Puro-12xMS2

Plasmid

#212628

Purpose

MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

Depositor

Paul Blainey

Publication

Najia et al Cell. 2026 Sep 1:S0092-8674(26)00932-3.

Insert

EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-KIR3DX1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156774

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR3DX1 (KIR3DX1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR-341 pUC19-tNGFR-P2A-PDCD1 N

Plasmid

#186074

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Publication

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PDCD1 (PDCD1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
px459-Rheb sgRNA

Plasmid

#133768

Purpose

Expresses Cas9 and human Rheb sgRNA

Depositor

Shawn Ferguson

Publication

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rheb sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge