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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pTH-iCre-WPREpA

Plasmid

#213130

Purpose

Truncated rat TH promoter expressing iCre

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-foldon-GFP- PCP

Plasmid

#194503

Purpose

Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Insert

foldon-GFP-PCP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd-V106W

Plasmid

#193839

Purpose

Express TadCBEd-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd V106W-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc

Plasmid

#223559

Purpose

Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 N-T2A-M-IRES-E

Plasmid

#231903

Purpose

For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)

Expression

Mammalian

Mutation

R203M in N protein

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_goldDn29-dCas9-P2A-GFP

Plasmid

#247167

Purpose

In vitro transcription of human codon optimized fusion of goldDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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ubbr:GCaMP8m

Plasmid

#232485

Purpose

To make fish transgenic lines that have calcium sensor expression in the whole body of the fish

Depositor

Chie Satou

Article

Ruetten et al bioRxiv 2025.08.20.670374

Insert

jGCaMP8m

Use

Zebrafish expression

Promoter

ubbr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufABCDSE

Plasmid

#195056

Purpose

Expresses SUF operon from E. coli from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufABCDSE

Expression

Bacterial

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufCDSUB

Plasmid

#195055

Purpose

Expresses SUF operon from B. subtilis from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufCDSUB

Expression

Bacterial

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2m

Plasmid

#208700

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CaV1.2/pcDNA3.1~ Human

Plasmid

#140576

Purpose

Expression in mammallian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

Insert

human CaV1.2 (CACNA1C Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6f

Plasmid

#128315

Purpose

Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1

Insert

GCaMP6f

Use

AAV

Tags

His Tag

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hNR2F2-RFP

Plasmid

#22926

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148862)

Plasmid

#80263

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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