We narrowed to 66,964 results for: nin
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Plasmid#8386DepositorInsertfloxed EGFP
UseCre/Lox; Beta-galExpressionMammalianAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
ExpressionBacterialAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5(TA)-ires-puro
Plasmid#167823Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
dTAG NLRP1
Plasmid#166825PurposedTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLRP1 (NLRP1 Human)
ExpressionMammalianPromoterhPGKAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163075PurposeEntry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertroGFP2-Orp1
UseGateway CloningAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HC87 pEYFPN1 anti-Homer1 nanobody
Plasmid#135223PurposeMammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
Tags6xHis-HA-EYFPExpressionMammalianAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-CMV-cGAS C396/7A-HA
Plasmid#130917PurposeExpresses human cGAS-HA with C396/397A point mutations; Puromycin selection markerDepositorInsertcGAS C396/7A
TagsHAExpressionMammalianMutationmutated residues 396 and 397 to alaninesPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP(V5-APEX2-NES)
Plasmid#107596PurposeExpresses V5-APEX2-NES in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2-NES
UseFrt site to insert into hek293 t-rex genomeTagsNES and V5ExpressionMammalianPromoterCMV Dox inducibleAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-B2AR-APEX
Plasmid#96848PurposeFor expression of B2AR-APEX in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB2AR-APEX (ADRB2 Human)
ExpressionMammalianAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorPublicationInsertsSpCas9
PmCDA1
TagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
hMYOG-3xHA-IRES-hrGFP (#324)
Plasmid#78341PurposeOverexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMYOG (MYOG Human)
Tags3x HA tag and IRES-hrGFPExpressionMammalianPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-HSF1Δ∼PRD
Plasmid#71725Purposeplasmid expressing mic-his tag phosphorylation-deficient hHSF1DepositorInsertphosphorylation-deficient HSF1 (HSF1 Human)
Tagsmac 6xHISExpressionMammalianMutation15 phosphorylation sites in RD mutated to AlanineAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 (delta110)
Plasmid#71861Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, missing first 110 amino acidsPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST40-MFRN2
Plasmid#50546PurposeExpression of MFRN2 gene in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMrfn2 (SLC25A28 Human)
TagsV5ExpressionMammalianAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-792-pGex2TK
Plasmid#21748DepositorPublicationInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-792 of wt Rb …Available sinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
HotPETase_pET21b
Plasmid#215407PurposeExpression construct for HotPETase gene, codon optimized for expression in E. coliDepositorInsertHotPETase
ExpressionBacterialMutationS121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by