167,825 results

1. PB CAG-sfGFP-T2A-iCreERT2

   Plasmid

   #177309

   Purpose

   CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Expression

   Mammalian

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-PM-RA-BlastR

   Plasmid

   #211708

   Purpose

   Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   PM-targetted RA (Lck Synthetic)
   

   Use

   Lentiviral

   Tags

   RA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

   Plasmid

   #183609

   Purpose

   Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   Neo
   mKate2-3xNLS
   

   Use

   Mouse Targeting

   Tags

   3xNLS

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAd/WT1-IRES-nAmCyan

   Plasmid

   #29756

   Depositor

   Edward McCabe

   Insert

   Wilms tumor 1 (WT1 Human)
   

   Tags

   AmCyan, IRES, and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   No mutations; AmCyan is nuclear localized; WT1 an…

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

   Plasmid

   #81253

   Purpose

   Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   TetR and LacI
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-Pu.1

   Plasmid

   #139839

   Purpose

   doxycycline-inducible expression of mouse Pu.1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Pu.1 (Spi1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pYC01

    Plasmid

    #224586

    Purpose

    IPTG-inducible phage φX174 lysis gene E

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    phage φX174 lysis gene E (phiX174p08 )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PA1lacO-1

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-8XGTIIC-DsRED-DD

    Plasmid

    #115798

    Purpose

    lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP responsive promoter (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    DsRED-DHFR

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD44P pGL3

    Plasmid

    #19122

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 promoter (CD44 Human)
    

    Use

    Non viral cloning vector

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    CalR-SNAP-tag2-KDEL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLBS-mScarlet

    Plasmid

    #129337

    Purpose

    cytosol marker, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_DLL1_WT

    Plasmid

    #82108

    Purpose

    Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    DLL1 (DLL1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MutaT7

    Bacterial Strain

    #156460

    Purpose

    An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Bacterial Resistance

    Streptomycin

    Species

    Rattus norvegicus

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pRN114

    Plasmid

    #127221

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, Wus, AtSTM
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUCmini-iCAP-AAV.MaCPNS1

    Plasmid

    #185136

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS1 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTETRIS-cargo

    Plasmid

    #126033

    Purpose

    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Type

    Empty backbone

    Use

    Luciferase; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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