We narrowed to 11,436 results for: 110

1. pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

   Plasmid

   #223160

   Purpose

   Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   dGFP-P2A-NanoLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1289-AAV-dCjCas9

   Plasmid

   #223138

   Purpose

   AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIS1-Eef25UTR-renilla

   Plasmid

   #38235

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2 5'UTR / Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Eef2 5' UTR fused to renilla ORF

   Promoter

   Endogenous Eef2

   Available since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Plagl2

   Plasmid

   #228061

   Purpose

   transiently express mouse Plagl2

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Plagl2 (Plagl2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-IC2-FL

   Plasmid

   #51409

   Purpose

   expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

   Depositor

   Trina Schroer

   Article

   King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

   Insert

   dynein intermediate chain IC2C (Dync1i2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.13/AUG-FFLuc-3XFLAG

   Plasmid

   #127333

   Purpose

   Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   Firefly luciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CV40

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNAHygro(+)LAMP2B

   Plasmid

   #86029

   Purpose

   Expresses LAMP2B in Mammalian cells

   Depositor

   Janice Blum

   Article

   Zhou et al Immunity. 2005 May;22(5):571-81.

   Insert

   Lysosome-associated membrane protein 2b (LAMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mApple-MyosinIIA-C-18

   Plasmid

   #54929

   Purpose

   Localization: Cytoskeleton, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

   Insert

   MyosinIIA
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. piggyFlex

   Plasmid

   #218234

   Purpose

   A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

   Depositor

   Jay Shendure

   Article

   Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

    Plasmid

    #229791

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM33_PDCD6IP_ALIX V-domain

    Plasmid

    #178483

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIS1-Eef25UTR-TOPmut-renilla

    Plasmid

    #38236

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    The first nucleotides of the 5' UTR were cha…

    Promoter

    Endogenous Eef2

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pDIRECT_10E

    Plasmid

    #91209

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

    Plasmid

    #220090

    Purpose

    Recruitable PIP5K to make PIP2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Use

    Tags

    TagBFP-FKBP1A(3-108)

    Expression

    Mammalian

    Mutation

    amino acids 1-635; D101R, R304D, R445E, K446E

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9 MDC123

    Plasmid

    #59184

    Purpose

    2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2X35S

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET-WHaloCaMP1a

    Plasmid

    #205304

    Purpose

    Expression of WHaloCaMP1a in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR-ARF19

    Plasmid

    #83846

    Purpose

    Gateway donor vector for ARF19

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    ARF19 (ARF19 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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