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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,934 results for: nol

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Showing: 8661 - 8680 of 15934 results

  3. pIMVAd39T

    Plasmid
    #98303
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Expression
    Yeast
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98303-plasmid-map-sequence-id-182779

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  6. pCosSAD1AprR

    Plasmid
    #190786
    Purpose
    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination
    Depositor
    Jan Kormanec
    Article
    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(
    Inserts
    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190786-plasmid-map-sequence-id-375582

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  16. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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  19. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  20. pOCC240

    Plasmid
    #118895
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and mCherry, clea…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118895-plasmid-map-sequence-id-232366

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Showing: 8661 - 8680 of 15934 results