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Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by
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lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Lyso-ExRai-AMPKAR
Plasmid#192449PurposeExRai-AMPKAR targeted to the lysosomal membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyso-ExRai-AMPKAR
TagsFull-length LAMP1ExpressionMammalianPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)
Plasmid#162230PurposeLentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα-EGFP
Plasmid#181840PurposeGFP-tagged PKA RIα subunit for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRIα-EGFP (PRKAR1A Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with EmGFP donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertemGFP
ExpressionMammalianPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneExpressionMammalianPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-SspB-mScarlet-I-MYPT169
Plasmid#178522PurposeExpresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSspB-mScarlet-I-MYPT169
ExpressionMammalianPromoterCAGAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-ChR2-mCherry (AAV1)
Viral prep#135634-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyo182
Plasmid#173136PurposeExpresses pheS_A294G in e.coliDepositorInsertpheS_A294G
ExpressionBacterialAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nrf1-Myc pcDNA3.1(+)-C-Myc
Plasmid#169125Purposeexpression of proteinDepositorAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff 2.0-ChR2-mCherry (AAV8)
Viral prep#137143-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-mCherry (#137143). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-mCherry plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsmCherry (Cre-dependent)Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 GFPExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmExRai-AKAR2
Plasmid#161755PurposePlasma membrane-targeted ExRai-AKAR2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-ExRai-AKAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRCA1 (BRCA1 Human)
ExpressionMammalianPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1
Plasmid#159128Purposeexpresses KEAP1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKEAP1 (KEAP1 Human)
Tags2X-FLAG _ 2X-STREPExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXJ1
Plasmid#153129PurposeExpression of Flag tagged human FOXJ1 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW (AAV1)
Viral prep#75469-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-Flp-DOG-NW (#75469). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flp-DOG-NW plasmid DNA. EF1a-driven expression of Flp recombinase dependent on GFP (Flp-DOG); to be coinjected with GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only