We narrowed to 18,340 results for: Mut

1. pMXS-IRES-BLAST flag-CAD R2187A

   Plasmid

   #169827

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXS-IRES-BLAST flag-CAD H1734A H1741A

   Plasmid

   #169830

   Purpose

   Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   H1734A H1741A; TCCC -> AGTC silent mutations a…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXS-IRES-BLAST flag-CAD T456A S1406A

   Plasmid

   #169823

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A; TCCC -> AGTC silent mutations at…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXS-IRES-BLAST flag-CAD T456A S1859A

   Plasmid

   #169824

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1859A; TCCC -> AGTC silent mutations at…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-N1303K-ngRNA+14_EF1a-puroR

   Plasmid

   #207359

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR +14 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-L227R-ngRNA+15_EF1a-puroR

   Plasmid

   #207360

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR +15 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sh2g-ZMYND8-human

   Plasmid

   #201406

   Purpose

   Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

   Insert

   ZMYND8 (ZMYND8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sh4p-ZMYND8-mouse

   Plasmid

   #201405

   Purpose

   Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

   Insert

   ZMYND8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFB-CG-LYCHOS FP>IA-HA-GFP

   Plasmid

   #199688

   Purpose

   Expression of LYCHOS FP>IA mutant in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Tags

   10xHis, 6xHis, EGFP, HA, and HRV 3C

   Expression

   Insect

   Mutation

   F43 and P44 mutated to I and A respectively

   Promoter

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD649-HAsp-CD4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156808

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pInducer 21-3xFlag-hMEFV-ΔPYD

    Plasmid

    #195478

    Purpose

    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminus

    Depositor

    Thomas Henry

    Article

    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    deletion of residues 1-92 of pyrin (the PYD domai…

    Promoter

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD

    Plasmid

    #194608

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of N-terminal CARD domain and changed Cy…

    Promoter

    CMV

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXS-IRES-BLAST flag-CAD G-Loop1

    Plasmid

    #188128

    Purpose

    Expresses C-terminal flag-tagged human CAD G-Loop1 in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMXS-IRES-BLAST flag-CAD G-Loop2

    Plasmid

    #188129

    Purpose

    Expresses C-terminal flag-tagged human CAD G-Loop2 in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mGFP-hNET-A457P

    Plasmid

    #193365

    Purpose

    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

    Depositor

    Harald Sitte

    Article

    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

    Insert

    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    

    Use

    Tags

    monomeric GFP (mGFP)

    Expression

    Mammalian

    Mutation

    A457P (GCC to CCC)

    Promoter

    CMV

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_W

    Plasmid

    #147898

    Purpose

    Mammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1-del1097-1110-NF1144AA-shRNAres (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation and one non silent mutation R23…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-EGFP-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W

    Plasmid

    #147899

    Purpose

    Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    HsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation and one non silent mutation R23…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV6-Entry-EPHX1c.337T>C-Flag

    Plasmid

    #169169

    Purpose

    Expresses EPHX1 c.337T>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.337T>C

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV6-Entry-EPHX1c.416A>G-Flag

    Plasmid

    #169170

    Purpose

    Expresses EPHX1 c.416A>G in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    c.337T>C

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV6-Entry-EPHX1c.997A>C-Flag

    Plasmid

    #169171

    Purpose

    Expresses EPHX1 c.997A>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.997A>C

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge