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pDONR221_SLC10A3

Plasmid

#131931

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A3 (SLC10A3 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35A5

Plasmid

#132111

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A5 (SLC35A5 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEN_TTmiRc2_BirA_CDKN1A

Plasmid

#136523

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CDKN1A (CDKN1A Human)

Use

Gateway Cloning

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A9

Plasmid

#132216

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A9 (SLC4A9 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pTAMAHISTEV_PfeAR480A-Q482A

Plasmid

#128946

Purpose

expresses R480A Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pURD214

Plasmid

#113871

Purpose

expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA5 HXT13-15-16 sgRNA2-HXT2

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR220

Plasmid

#113873

Purpose

expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA8-HXT10 sgRNA7-HXT9-11-12

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR295

Plasmid

#113874

Purpose

expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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GST-ENLYFQC-PNT(46-138)-His6

Plasmid

#42919

Depositor

Barbara Imperiali

Article

Lukovic et al Angew Chem Int Ed Engl. 2009;48(37):6828

Insert

Ets-1 PNT domain (46-138) (Ets1 Mouse)

Tags

6xHis, GST, and TEV protease cleavage site

Expression

Bacterial

Mutation

Contains the PNT domain (Serine 46 through Lysine…

Promoter

Ptac

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
zgc:153086_R (OZ512)

Plasmid

#27187

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:153086

Use

Zebrafish targeting

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
SLC30A10-delta105-107

Plasmid

#65205

Purpose

Constitutive expression of SLC30A10 delta 105-107 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Leyva-Illades et al J Neurosci. 2014 Oct 15;34(42):14079-95.

Insert

SLC30A10 (SLC30A10 Human)

Tags

Flag

Expression

Mammalian

Mutation

delta 105-107 amino acids

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500

Plasmid

#92316

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NDUFS6(5’UTR)-FF

Plasmid

#85488

Purpose

Firefly luciferase under the control of NDUFS6 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of NDUFS6 (NDUFS6 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 H60A

Plasmid

#71863

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Histidine 60 mutated to Alanine

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60-FLAG-SLC38A9.1 L74A

Plasmid

#71866

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Leucine 74 mutated to Alanine

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60-FLAG-SLC38A9.1 P90A

Plasmid

#71867

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Proline 90 mutated to Alanine

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
zgc:153086_L (OZ511)

Plasmid

#27186

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:153086

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
SDC3_L (OZ595)

Plasmid

#35235

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
SDC3_R (OZ596)

Plasmid

#35236

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only