169,450 results
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Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
Tags3X Flag TagExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STAT3_WT
Plasmid#82235PurposeGateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUltra-U6-gRNAs1-5
Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL_PylRS(WT)-tRNA
Plasmid#223512PurposePylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsTags6xHisExpressionBacterialPromoteraraBAD, rrnB, and glnS and proKAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6e
Plasmid#207855PurposeMammalian expression of PE6e prime editorDepositorInsertPE6e
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, K918A, K775R)Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAKTaq
Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
ExpressionBacterialAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only