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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,056 results for: Met

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Showing: 8761 - 8780 of 14056 results

  4. pRRL-VinculinVenus-S1033D

    Plasmid
    #211897
    Purpose
    Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Publication
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinVenus-S1033D (VCL Chicken)
    Use
    Lentiviral
    Tags
    VenusA206K
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211897-plasmid-map-sequence-id-419258

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  5. pETSUMO2_Vitiosangium-bGSDM

    Plasmid
    #212127
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Vitiosangium bGSDM
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212127-plasmid-map-sequence-id-419098

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  7. anti-VGluT1 scFv [N28/9] with sortase tag

    Plasmid
    #204424
    Purpose
    Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.
    Depositor
    Jeff Lichtman
    Publication
    Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:
    Insert
    Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    204424-plasmid-map-sequence-id-406613

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  10. pPT-TAR C1

    Plasmid
    #141246
    Purpose
    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1
    Depositor
    Bogumil Karas
    Publication
    Cochrane et al Algal Research, 2020. 50, 102014
    Insert
    Mitochondrial Genome of Phaeodactylum tricornutum
    Use
    Synthetic Biology; Diatom expression
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    141246-plasmid-map-sequence-id-367010

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  11. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Publication
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199222-plasmid-map-sequence-id-396540

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  16. pDEST27-GST-hADAM10

    Plasmid
    #189553
    Purpose
    Protein expression ADAM10 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    ADAM metallopeptidase domain 10 (ADAM10 Human)
    Tags
    GST
    Expression
    Mammalian
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    189553-plasmid-map-sequence-id-384138

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  17. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid
    #194169
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Publication
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Tags
    3X flag
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194169-plasmid-map-sequence-id-381800

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Showing: 8761 - 8780 of 14056 results