We narrowed to 10,634 results for: SEC
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Plasmid#186655PurposeDonor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
Tags3xFlag-3xHAExpressionInsectAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_Nbr
Plasmid#186662PurposeDonor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.DepositorInsertOSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_N_FH_GLE
Plasmid#186657PurposeDonor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R
Plasmid#167173PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cellsDepositorInsertsATP1A1-S+Q111R
ATP1B1
ExpressionBacterial and InsectMutationChanged Q at 111 to RPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+N122D
Plasmid#167174PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cellsDepositorInsertsATP1A1-S+N122D
ATP1B1
ExpressionBacterial and InsectMutationChanged N at 111 to DPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY2
Plasmid#166712Purposeexpresses AMOT with inactivating mutation in second PPxY motif in mammalian cellsDepositorInsertAngiomotin p130 P262A P263A (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged proline 262 to alanine, proline 263 to al…PromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
BE3-YE1
Plasmid#132943PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-(W90Y-R126E)
UseCRISPR; Base editorExpressionMammalianMutationBE3-(W90Y-R126E)Available SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N entry vector
Plasmid#111751PurposeIntermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cellsDepositorTypeEmpty backboneUseBaculovirus expressionTagsFLAGExpressionMammalianAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
TagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
TagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
TagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A C-Term
Plasmid#69795PurposePebble isoform A, C-Term, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble C-Term domain (Drosophila guanine nucleotide exchange factor)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationJust the C-Term domain of Pebble (pbl)Promoterhsp70 promoterAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
RF21
Bacterial Strain#102803PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)DepositorBacterial ResistanceNoneAvailable SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA4A-COMP5AP-AviTag-9xHis
Plasmid#157502PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_KLF4
Plasmid#26815DepositorAvailable SinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1
Plasmid#159576PurposeBaculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRMutationXbaI site added upstream of U6 promotor. GtoC mu…Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ITSN-mcherry-sspB
Plasmid#85220PurposeRole of Cdc-42 selective GEF domain from ITSN in immune cell migrationDepositorInsertsTagsNAExpressionMammalianMutationR73QPromoterCMVAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis
Plasmid#157187PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
TagsHistidineExpressionBacterial, Insect, and Mamm…PromoterCMV ie enhancer/promoterAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only