We narrowed to 9,775 results for: Tec;

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pmCherry-AHPH

Plasmid

#187278

Purpose

Express mCherry-AHPH (RhoA biosensor)

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin and Pleckstrin homology domain (ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AHPH

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Tags

6x HIS

Expression

Insect

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES2_hMela(CT+IL3 mGluR6)

Plasmid

#191343

Purpose

Optogenetic tool

Depositor

Sonja Kleinlogel

Article

Kralik et al Commun Biol. 2022 Oct 20;5(1):1116. doi:

Insert

hMela(CT+IL3 mGluR6)

Tags

IRES_Turbo

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K246A

Plasmid

#192051

Purpose

Expresses a catalytic mutant of SOX (K246A)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K246A)

Use

Unspecified

Mutation

Catalytic mutant K246A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K250A

Plasmid

#192052

Purpose

Expresses a DNA binding mutant K250A (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K250A)

Use

Unspecified

Mutation

DNA binding mutant K250A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHsp70(K71E)-GFP

Plasmid

#228196

Purpose

expressed human Hsp70 (K71E mutation) with a GFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

French et al Proc Natl Acad Sci U S A. 2013 Feb 12;11

Insert

HSP70(K71E) (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Mutation

K71E

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-chimpanzee cGAS-HA

Plasmid

#186873

Purpose

Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Chimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NS3/4A S139A

Plasmid

#186885

Purpose

Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

HCV NS3/4A protease with S139A mutation

Use

Retroviral

Mutation

S139A

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-WHG cGAS-HA

Plasmid

#186867

Purpose

Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

White-handed gibbon cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-orangutan cGAS-HA

Plasmid

#186871

Purpose

Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Orangutan cGAS with C-terminal HA tag (CGAS Pongo abelii)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

Plasmid

#194233

Purpose

Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

Plasmid

#194234

Purpose

Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MYD88-ts3

Plasmid

#174282

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MYD88-ts1

Plasmid

#174280

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MYD88-ts2

Plasmid

#174281

Purpose

MYD88 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MYD88 shRNA (MYD88 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-MOV10-ts3

Plasmid

#174279

Purpose

MOV10 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MOV10 shRNA (MOV10 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-HNRNPR-ts3

Plasmid

#174255

Purpose

HNRNPR knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HNRNPR shRNA (HNRNPR Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-IFI16-ts1

Plasmid

#174256

Purpose

IFI16 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

IFI16 shRNA (IFI16 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-IFI16-ts2

Plasmid

#174257

Purpose

IFI16 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

IFI16 shRNA (IFI16 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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