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We narrowed to 111,015 results for: cloning

Showing: 8761 - 8780 of 111015 results
  1. pPCR-Script-mLIP

    Plasmid
    #49194
    Purpose
    initial cloning vector for mLIP fragment of C/EBPbeta
    Depositor
    Insert
    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    Use
    Cloning vector
    Expression
    Bacterial
    Mutation
    only the LIP fragment
    Promoter
    lac
    Available Since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. ARL5:H14-K177 (2H16)

    Plasmid
    #25526
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    ARL5:H14-K177 (ARL5A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids H14-K177
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pGEX6P1-DEST-His

    Plasmid
    #119763
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 8761 - 8780 of 111015 results