We narrowed to 27,858 results for: sta

1. pGGA016

   Plasmid

   #228231

   Purpose

   Level 0 for 5xOp (GreenGate A module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   5xOp
   

   Use

   Synthetic Biology

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGC018

   Plasmid

   #228232

   Purpose

   Level 0 for LhG4 (GreenGate C module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   GR-LhG4
   

   Use

   Synthetic Biology

   Promoter

   -

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB-CRISPRi

   Plasmid

   #221136

   Purpose

   Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   3xF-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial

   Promoter

   cbu1169 promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-T7-VHH-C9BoNT-A

   Plasmid

   #202490

   Purpose

   Expresses the nanobody C9 BoNT/A with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody C9 BoNT/A
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-Amp-T7-GFPuv

   Plasmid

   #202463

   Purpose

   Expresses GFPuv with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. insulator_seq_UENU_donor

   Plasmid

   #221459

   Purpose

   Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

   Depositor

   Maria Cristina Gambetta

   Article

   Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

   Insert

   3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
   

   Use

   Synthetic Biology

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. insulator_seq_reporter_vector

   Plasmid

   #221460

   Purpose

   Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.

   Depositor

   Maria Cristina Gambetta

   Article

   Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

   Insert

   gypsy insulator-EGFP-neutral spacer DNA-mCherry
   

   Use

   Synthetic Biology

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dm_λHA-FLuc100_CSC

    Plasmid

    #210592

    Purpose

    Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)
    

    Tags

    lambda HA

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dm_Opt_Renilla_CSC

    Plasmid

    #210593

    Purpose

    Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)
    

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB_OptλHAramp-FLuc100

    Plasmid

    #210577

    Purpose

    Expresses Optimal lambda HA-Optimal Firefly luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Optimal lambda HA-Optimal Firefly luciferase
    

    Tags

    lambda HA

    Expression

    Mammalian

    Promoter

    UBC human

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUB_NonoptλHAramp-FLuc100

    Plasmid

    #210579

    Purpose

    Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Nonoptimal lambda HA-Optimal Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    lambda HA

    Expression

    Mammalian

    Promoter

    UBC human

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_SM-FLuc100-pA

    Plasmid

    #210584

    Purpose

    Expresses SM(FLAG)-optimal Firefly luciferase - pA

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dm_λHA-FLuc0_CSC

    Plasmid

    #210589

    Purpose

    Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
    

    Use

    Luciferase

    Tags

    lambda HA

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. POSV801-SP44-SnaABCDEOinT1T2

    Plasmid

    #225058

    Purpose

    Heterologous expression in Streptomyces with inactive SnaO

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    inactivated SnaO
    

    Expression

    Bacterial

    Mutation

    deltaR51-V511

    Promoter

    SP44

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. POSV801-SP44-SnaABCDEOT1T2

    Plasmid

    #225055

    Purpose

    Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    SnaA
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    SP44

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA_CloningVector_tagBFP

    Plasmid

    #163747

    Purpose

    Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNA_CloningVector_miRFP670

    Plasmid

    #163748

    Purpose

    Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MDH1_KW

    Plasmid

    #224145

    Purpose

    IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

    Depositor

    Katherine Walstrom

    Article

    Thomas et al Biochim Biophys Acta Proteins Proteom. 2

    Insert

    MDH-1 (F46E10.10 Nematode)
    

    Tags

    Ala-intein-CBD

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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