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AiP1870 - pAAV-AiE2492m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230724

Purpose

AiE2492m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1872 - pAAV-AiE2517m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230725

Purpose

AiE2517m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1504 - pAAV-AiE2187m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230663

Purpose

AiE2187m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1187 - pAAV-AiE2046m-minBG-FlpO-WPRE-HGHpA

Plasmid

#230578

Purpose

AiE2046m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1871 - pAAV-AiE2515m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#220677

Purpose

AiE2515m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1878 - pAAV-AiE2425m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214566

Purpose

AiE2425m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1873 - pAAV-AiE2499m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214564

Purpose

AiE2499m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1876 - pAAV-AiE2413m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214565

Purpose

AiE2413m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP1879 - pAAV-AiE2570m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214567

Purpose

AiE2570m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDAX-187 N-Tag GFP[1] L10 FKBP mCherry

Plasmid

#130003

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

Plasmid

#107847

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

All asparagine to glutamine in splicing reporter

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

Plasmid

#223162

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-YFP-Smo

Plasmid

#31618

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

YFP

Expression

Mammalian and Worm

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-SNAP-Smo

Plasmid

#31617

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

SNAP

Expression

Mammalian and Worm

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMO819

Plasmid

#232072

Purpose

Expresses Cytosine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Cytosine Base Editor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1-alpha core promoter

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Article

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1-alpha core promoter

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1_SEPT2

Plasmid

#71549

Purpose

mammalian expression of Sept2 with an mCherry fusion.

Depositor

Elias Spiliotis

Article

Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

Insert

Septin 2 (Septin2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Arf Q71L CFP

Plasmid

#128149

Purpose

Expression of Arf Q71L

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 2002 May 9;417(6885):187-93. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Q71L

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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