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pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(G137I)

Plasmid

#133274

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G137I

Promoter

CMVd2

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2/1

Plasmid

#112862

Purpose

AAV packaging plasmid expressing Rep/Cap genes

Depositor

James M. Wilson

Insert

Rep2/Cap1

Use

AAV

Promoter

Truncated P5

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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sgMST1/2-1

Plasmid

#229429

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-1 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMALac-OMA-1(1-182)

Plasmid

#80350

Purpose

Expresses OMA-1 (1-182) in bacteria for protein purification purposes

Depositor

Sean Ryder

Article

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

OMA-1 (oma-1 Nematode)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Encodes AA residues 1-182

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNSU299_CUP1-1

Plasmid

#166086

Purpose

Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast

Depositor

Rebecca Burgess

Insert

CUP1-1 (CUP1-1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

Tet-inducible

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shPRDM14-1

Plasmid

#193694

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_TJP1-1/1-SV2/2

Plasmid

#91494

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV2/2 (TJP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TJP1-1/1-SV1/2

Plasmid

#91321

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV1/2 (TJP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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NH-1

Plasmid

#87506

Purpose

For library amplification of fragment NH-1

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NH-1

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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NI-1

Plasmid

#87474

Purpose

For library amplification of fragment NI-1

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NI-1

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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NG-1

Plasmid

#87490

Purpose

For library amplification of fragment NG-1

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

NG-1

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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HD-1

Plasmid

#87458

Purpose

For library amplification of fragment HD-1

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Insert

HD-1

Use

Synthetic Biology and TALEN; Tale dbd library pre…

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV1/3

Plasmid

#91548

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV1/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOSTRIN-1/1-SV1/2

Plasmid

#91323

Purpose

Protein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOSTRIN-1/1-SV1/2 (NOSTRIN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MPP4-1/1-IS3/3

Plasmid

#91519

Purpose

Protein expression and purification of human SH3 domain construct MPP4-1/1-IS3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP4-1/1-IS3/3 (MPP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV3/3

Plasmid

#91451

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV3/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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