Skip to main content
Addgene
Showing: 861 - 880 of 82032 results
  1. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. Hygro-iDEST

    Plasmid
    #75339
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 861 - 880 of 82032 results