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We narrowed to 4,840 results for: donor

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Showing: 861 - 880 of 4840 results

  3. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    131461-plasmid-map-sequence-id-305184

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  7. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid
    #80769
    Purpose
    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).
    Depositor
    Kazuhisa Nakayama
    Publication
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFT88 gRNA#1 (IFT88 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80769-plasmid-map-sequence-id-171039

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  12. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Publication
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    162277-plasmid-map-sequence-id-319794

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  16. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  19. pHiUGE smFP-V5 donor ORF0

    Plasmid
    #200380
    Purpose
    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
    Depositor
    Scott Soderling
    Publication
    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    Expression
    Mammalian
    Mutation
    NA
    Available since
    July 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    200380-plasmid-map-sequence-id-397388

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  20. pHiUGE smFP-V5 donor ORF1

    Plasmid
    #200381
    Purpose
    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal
    Depositor
    Scott Soderling
    Publication
    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    Expression
    Mammalian
    Mutation
    NA
    Available since
    July 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    200381-plasmid-map-sequence-id-397389

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Showing: 861 - 880 of 4840 results