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Plasmid#112018PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFPDepositorInsertCD4-GFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-mCherry HDRT Source (pTR 144)
Plasmid#112013PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherryDepositorInsertRAB11A-mCherry HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-mCherry HDRT Source (pTR 177)
Plasmid#112017PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherryDepositorInsertCLTA-mCherry HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
BATF-GFP HDRT Source (pTR 146)
Plasmid#112015PurposeDNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFPDepositorInsertBATF-GFP HDRT (BATF Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB11A-BFP HDRT Source (pTR 145)
Plasmid#112014PurposeDNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFPDepositorInsertRAB11A-BFP HDRT (RAB11A Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-BFP HDRT Source (pTR 176)
Plasmid#112020PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFPDepositorInsertCD4-BFP HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4-C-mCherry HDRT Source (pTR 175)
Plasmid#112019PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherryDepositorInsertCD4-mCherry HDRT (CD4 Human)
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
PG13-mCherry
Plasmid#195291PurposemCherry reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PG13-mCherry-EF1a-PuroR-EGFP
Plasmid#195292PurposepiggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)DepositorInsertsmCherry
PuroR-P2A-EGFP
UsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNIL
Plasmid#172113PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expressionUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB2.1
Plasmid#40728PurposeModified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert backgroundDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
UseTagsExpressionMammalianMutationTol2 codon usage frequency well suitable for muriā¦PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorInsertSPHK2 (SPHK2 Human)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
Plasmid#61255PurposeCRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-sTomato-NLS-blast
Plasmid#136259PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only