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We narrowed to 1,795 results for: nif

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Showing: 861 - 880 of 1795 results

  1. pTet_KaeCanABC(G104K,G189K)

    Plasmid
    #248958
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G104K,G189K)
    Expression
    Bacterial
    Mutation
    G104K,G189K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248958-plasmid-map-sequence-id-500779

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  2. pTet_PflHamMA(-cJun)B

    Plasmid
    #248940
    Purpose
    Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker
    Depositor
    Jennifer Doudna
    Insert
    flHamMA(-cJun)B
    Expression
    Bacterial
    Mutation
    c-Jun insertion, HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248940-plasmid-map-sequence-id-500774

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  3. pLG017_FLAG-Avs1a(I134K,I234K)

    Plasmid
    #248949
    Purpose
    AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K)
    Depositor
    Jennifer Doudna
    Insert
    FLAG-Avs1a(I134K,I234K)
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248949-plasmid-map-sequence-id-500770

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  4. pTet_PflHamM(I118K,I218K)AB

    Plasmid
    #248937
    Purpose
    Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K)
    Depositor
    Jennifer Doudna
    Insert
    PflHamM(I118K,I218K)AB
    Expression
    Bacterial
    Mutation
    I118K,I218K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248937-plasmid-map-sequence-id-500765

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  5. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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  6. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219689-plasmid-map-sequence-id-435724

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  7. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  8. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

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  9. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  10. L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219687
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219687-plasmid-map-sequence-id-435722

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  11. pTet_PflHamMA(S157A)(deltaA)B

    Plasmid
    #248939
    Purpose
    Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation
    Depositor
    Jennifer Doudna
    Insert
    PflHamMA(S157A)(deltaA)B
    Expression
    Bacterial
    Mutation
    S157A, HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248939-plasmid-map-sequence-id-500773

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  12. L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10

    Plasmid
    #219699
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH::PhiC31:tUBQ1 - pMUTE::Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219699-plasmid-map-sequence-id-435730

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  13. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

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  14. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219686-plasmid-map-sequence-id-436048

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  15. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid
    #219711
    Purpose
    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219711-plasmid-map-sequence-id-435738

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  16. pOpen-ECOligA

    Plasmid
    #165564
    Purpose
    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165564-plasmid-map-sequence-id-340043

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  18. TGN38 EGFP

    Plasmid
    #128148
    Purpose
    Expression of TGN38 amino acids 304-357
    Depositor
    Jennifer Lippincott-Schwartz
    Insert
    TGN38 (Tgoln2 Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Contains amino acids 304-357
    Available Since
    Nov. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128148-plasmid-map-sequence-id-251067

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  19. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  20. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

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Showing: 861 - 880 of 1795 results