We narrowed to 6,524 results for: pcDNA3.1+

1. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Use

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-OTUB1 WT-pcDNA3.1

   Plasmid

   #118209

   Purpose

   Expresses FLAG-OTUB1 WT in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1-Clover-mRuby2

   Plasmid

   #49089

   Purpose

   Clover-mRuby2 tandem fusion for FRET calibration

   Depositor

   Kurt Beam

   Insert

   Clover-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-300(HAT-)

   Plasmid

   #23254

   Depositor

   Warner Greene

   Article

   Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H1415A E1423A Y1424A L1428S Y1430A H1434A

   Promoter

   Available since

   March 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. N370S GBA pcDNA3.1

    Plasmid

    #188583

    Purpose

    Ectopic expression N370S GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N370S

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. E326K GBA pcDNA3.1

    Plasmid

    #188581

    Purpose

    Ectopic expression E326K GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E326K

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+) EGFP-Rac1(WT)

    Plasmid

    #224278

    Purpose

    Cell transfection and expression of Rac1

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. K44A HA-dynamin 2 pcDNA3.1

    Plasmid

    #34685

    Depositor

    Sandra Schmid

    Insert

    dynamin 2ba
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-GRP78/BiP

    Plasmid

    #32701

    Depositor

    Richard C. Austin

    Article

    Werstuck et al J Clin Invest. 2001 May;107(10):1263-73.

    Insert

    GRP78/BiP (HSPA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

    Plasmid

    #159130

    Purpose

    expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-(empty)-TAG

    Plasmid

    #138209

    Purpose

    To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Type

    Empty backbone

    Use

    T7 transcription

    Tags

    TAG sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter, T7 Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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