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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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anti-VGluT1 scFv [N28/9] with sortase tag

Plasmid

#204424

Purpose

Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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anti-PSD-95 scFv [K28/43] with sortase tag

Plasmid

#204422

Purpose

Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting PSD-95 (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1-5_Xt

Plasmid

#217132

Purpose

Mammalian expression vector for expression of Tc1-5_Xt transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1-5_Xt transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tc1-8B_DR

Plasmid

#217117

Purpose

Mammalian expression vector for expression of Tc1-8B_DR transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

Tc1-8B_DR transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-5_Xt

Plasmid

#217060

Purpose

Donor plasmid of Tc1-5_Xt Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-5_Xt transposon

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS

Plasmid

#216830

Purpose

cardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

PALMDgRNA

Use

AAV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pENAAV-cTNT-actn2-BioID2-HA

Plasmid

#216831

Purpose

cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

BioID-HA (Actn2 Synthetic)

Use

AAV

Tags

HA

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-MCAK-C-7

Plasmid

#56168

Purpose

Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MCAK (KIF2C Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-CBP HAT

Plasmid

#179547

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Tags

N-terminal mScarlet tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pICOZ-MAG-M235Q

Plasmid

#218299

Purpose

Mammalian expression vector for expression of MAG transposase mutant

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

M235Q mutant of MAG transposase

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-hAT-7_PM

Plasmid

#217147

Purpose

Mammalian expression vector for expression of hAT-7_PM transposase (CMV promoter)

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

hAT-7_PM transposase

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-hAT-7_PM

Plasmid

#217075

Purpose

Donor plasmid of hAT-7_PM Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of hAT-7_PM transposon

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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RANLS-LEHD-BNES

Plasmid

#60973

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A and ddRFP B

Tags

Caspase9 substrate sequence (LEHD) is after NLS s…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
p-miR-Report + WT miR-181 BS in GATA6 3UTR

Plasmid

#22794

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only