We narrowed to 37,143 results for: Tec

1. PIM1 gRNA (BRDN0001146465)

   Plasmid

   #77174

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PIM1 gRNA (BRDN0001146597)

   Plasmid

   #77175

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PIM1 gRNA (BRDN0001145078)

   Plasmid

   #77176

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIM1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIM1 (PIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Polymerase expression - pCMV-B103 (LM2907)

   Plasmid

   #208968

   Purpose

   Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-ms-Klf1

   Plasmid

   #50785

   Purpose

   retroviral expression of mouse Klf1

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Klf1 (Klf1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CNNM3-CBS-YPet

   Plasmid

   #149686

   Purpose

   For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

   Depositor

   Motoyuki Hattori

   Article

   Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

   Insert

   CNNM3-CBS-YPet (CNNM3 Human, Synthetic)
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA BRCA2 V572I

   Plasmid

   #139321

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 V572I using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. WEE1 gRNA (BRDN0001145570)

   Plasmid

   #77368

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human WEE1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   WEE1 (WEE1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pv9_MBD1_MBD-WT-13XL-BASU

   Plasmid

   #179416

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1.MBD-WT-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ROCK1 gRNA (BRDN0001147709)

    Plasmid

    #77593

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid

    #123346

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-CKAR
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. non-targeting control gRNA (BRDN0001145869)

    Plasmid

    #80256

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC39A14

    Plasmid

    #132105

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Zebrafish-gRNA-0004

    Plasmid

    #42244

    Purpose

    gRNA targeted to zebrafish gene fh [site #2]

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-fh site #2 (fh Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

15. [pSK483] pLJC5 Flag-Depdc5(P)

    Plasmid

    #109342

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant P

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    YDLLP(775-779)GSGSG

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PLK1 gRNA (BRDN0001145759)

    Plasmid

    #76457

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ROCK1 gRNA (BRDN0001145904)

    Plasmid

    #77594

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ROCK1 gRNA (BRDN0001146798)

    Plasmid

    #77595

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROCK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROCK1 (ROCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR CMVie-Lifeact-iRFP670 L1-R5

    Plasmid

    #84390

    Purpose

    Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Gateway

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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