We narrowed to 16,117 results for: grna

Showing: 8841 - 8860 of 16117 results

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sgRNA scaffold-spacer-human U6

Plasmid

#214697

Purpose

provide "sgRNA scaffold-spacer-human U6" fragment

Depositor

Yin Shen

Publication

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

sgRNA scaffold-spacer-human U6

Use

CRISPR

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-nicking sgRNA

Plasmid

#169860

Purpose

SauCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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SauCas9KKH CCR5-nicking sgRNA

Plasmid

#169863

Purpose

SauCas9KKH nicking sgRNA for CCR5

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9KKH CCR5-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Expression

Yeast

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 ROSA sgRNA mCherry

Plasmid

#155280

Purpose

Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

Depositor

Martin Carroll

Insert

spCas9

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCF820_U6-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138316

Purpose

Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Publication

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

Plasmid

#188703

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

Depositor

David Tobin

Publication

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgRNA_NUDT5_ex4-EF1α-Cas9-NLS-FLAG-GSG-P2A-PuroR-WPRE

Plasmid

#250374

Purpose

Lentiviral expression of sgRNA against human NUDT5 exon 4 under U6 promoter and Cas9-NLS-FLAG-GSG-P2A-PuroR under EF1α core promoter.

Depositor

Stefan Kubicek

Publication

Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

Insert

sgNUDT5 (NUDT5 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223166

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Publication

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170815

Purpose

Lentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Publication

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mScarlet-I-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Publication

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Expression

Mammalian

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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gh161

Plasmid

#106825

Purpose

expression of gRNA targeting TMTC2

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC2 (TMTC2 Human)

Expression

Mammalian

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh114

Plasmid

#106784

Purpose

expression of gRNA targeting B4GALNT2

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALNT2 (B4GALNT2 Human)

Expression

Mammalian

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh25

Plasmid

#106706

Purpose

expression of gRNA targeting POMGNT1

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POMGNT1 (POMGNT1 Human)

Expression

Mammalian

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh89

Plasmid

#106760

Purpose

expression of gRNA targeting FUT5

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT5 (FUT5 Human)

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh168

Plasmid

#106830

Purpose

expression of gRNA targeting A4GNT

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

A4GNT (A4GNT Human)

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh105

Plasmid

#106775

Purpose

expression of gRNA targeting B3GALT1

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT1 (B3GALT1 Human)

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh249

Plasmid

#106856

Purpose

expression of gRNA targeting PIGV

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

PIGV (PIGV Human)

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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