We narrowed to 28,328 results for: crispr grnas

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pSCAR_sgRNA_blast-tagBFP-lox2272

Plasmid

#162071

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

Plasmid

#167921

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

Plasmid

#167921

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Blasto_TtoC and hairpin extension

Plasmid

#167920

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA Blasto_TtoC and hairpin extension

Plasmid

#167920

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB700 lenti gRNA EYFP_TtoC and hairpin extension

Plasmid

#167922

Purpose

Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge