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pPD108 VP16-ZF43(RAAR)

Plasmid

#138813

Purpose

Constitutive expression of VP16-ZF43(RAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(RAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-TEV-GFP-His8

Plasmid

#250427

Purpose

Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker; Δ257-271) in Mammalian Cells

Depositor

Simon Newstead

Insert

PQLC1 (SLC66A2 Human)

Tags

TEV-GFP-8xHis

Expression

Mammalian

Mutation

Δ100-125 (Replaced with GGGS), Δ257-271

Promoter

Chicken-beta-actin promoter/CMV Enhancer

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLexM-HsPQLC1(Δ100-125/3xGGGS/Δ257-271)-TEV-GFP-His8

Plasmid

#250429

Purpose

Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by 3xGGGS Linker; Δ257-271) in Mammalian Cells

Depositor

Simon Newstead

Insert

PQLC1 (SLC66A2 Human)

Tags

TEV-GFP-8xHis

Expression

Mammalian

Mutation

Δ100-125 (Replaced with 3xGGGS), Δ257-271

Promoter

Chicken-beta-actin promoter/CMV Enhancer

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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R701-X57-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L

Plasmid

#159565

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X19-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V

Plasmid

#159570

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X20-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C

Plasmid

#159568

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X24-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Plasmid

#159573

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X23-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Plasmid

#159572

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Plasmid

#159566

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJB038 pAAV EF1a flox(mCherry)-EGFP

Plasmid

#252771

Purpose

An AAV vector that expresses a Cre-dependent mCherry to eGFP

Depositor

Joshua Leonard

Article

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

flox(mCherry)-EGFP

Use

AAV

Promoter

hEF1a

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLN491 (FuGW-G8p-CCL21-1Pe)

Plasmid

#105193

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

Plasmid

#105202

Purpose

Module 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLN553 (FuGW-S(MAFK)p-Sponge7)

Plasmid

#105203

Purpose

Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

Plasmid

#236421

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (Keap1 Mouse)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

Plasmid

#236422

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (KEAP1 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:D127-E814 (EIF3B Human)

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_DCAF11:1-546

Plasmid

#210907

Purpose

Insect expression of full-length human DCAF11. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF11:M1-Q546 (DCAF11 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

R207H natural variant (VAR_020121)

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHIE822 pCMV Bglobin intron VSVGmut K47Q R354A in pMD2

Plasmid

#252774

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Joshua Leonard

Article

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

VSVG K47Q R354A

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pHIE963 pCCL pSFFV Cre_BC_entry_IRES_PuroR

Plasmid

#252775

Purpose

3rd generation lentiviral vector encoding Cre recombinase

Depositor

Joshua Leonard

Article

Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70

Insert

Cre_BC_entry_IRES_PuroR

Use

Lentiviral

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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