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pFSynW Syt1 D303G IRES mRuby3

Plasmid

#170640

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

D303G

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 D365E IRES mRuby3

Plasmid

#170641

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

D365E

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D303G

Plasmid

#170646

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) D365E

Plasmid

#170649

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Syt1 C2AB (96-421) I367T

Plasmid

#170652

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available Since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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mApple-H2B-6

Plasmid

#54908

Purpose

Localization: Nucleus/Histones, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

H2B (H2BC11 Human)

Tags

mApple

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pGEM cCol2 (CT#617)

Plasmid

#13943

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Collagen II (COL2A1 Chicken)

Expression

Bacterial

Mutation

Nucleotides 182-616

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ER-Ras-LOCKR-S

Plasmid

#216500

Purpose

FRET-based reporter for Ras activity, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-IT6

Plasmid

#66870

Purpose

Inntag 6: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 6

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pOZC-PALB2

Plasmid

#133552

Purpose

PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

Depositor

David Livingston

Article

Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

Insert

PALB2 (PALB2 Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_crU626

Plasmid

#191776

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 N17

Plasmid

#15904

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

T17N

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
GFP-PICK

Plasmid

#31613

Depositor

Robert Malenka

Article

Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

Insert

PICK1 (shRNA insensitive) +shRNA towards PICK1 (Pick1 Rat)

Use

Lentiviral

Tags

GFP, HA, and PICK1 shRNA

Expression

Mammalian

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM cAgg (CT#689)

Plasmid

#13945

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Aggrecan (ACAN Chicken)

Expression

Bacterial

Mutation

Nucleotides 113-800

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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