166,671 results

1. pAC-BETA

   Plasmid

   #53272

   Purpose

   Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

   Insert

   crtE, crtY, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SV40-eGFP-Z1

   Plasmid

   #127638

   Purpose

   Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

   Depositor

   Jordan Green

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

   Plasmid

   #221225

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA M6-UGI-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-mCherry-flex-dtA

   Plasmid

   #58536

   Purpose

   Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

   Depositor

   Naoshige Uchida

   Article

   Wu et al Nature. 2014 May 15;509(7500):325-30. do

   Insert

   dtA (DIP_RS18250 )
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OMMGFP 1-10

   Plasmid

   #164119

   Purpose

   Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   OMMGFP 1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-HA-Ubiquitin-KO

   Plasmid

   #17603

   Purpose

   Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   KO: all lysines mutated to arginines

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIG-601_NY-ESO-1_TCR_GFP

   Plasmid

   #207482

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   GFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Potential silent point mutation in GFP sequence

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB-rtTA

   Plasmid

   #126034

   Purpose

   PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

   Depositor

   Mauro Calabrese

   Article

   Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

   Insert

   rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbiC

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTRK892

   Plasmid

   #71803

   Purpose

   Expression of mutated L. gasseri gusA from strong Ppgm promoter

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

   Insert

   gusA3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D573A

   Promoter

   Ppgm

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge