We narrowed to 15,730 results for: NOL

1. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

   Plasmid

   #160574

   Purpose

   AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:AsCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2 AsCas12a (GB1438)

   Plasmid

   #160572

   Purpose

   Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. IBMc227

   Plasmid

   #161948

   Purpose

   Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. IBMc367

   Plasmid

   #161947

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. IBMc228

   Plasmid

   #161946

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
   

   Expression

   Bacterial

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-Basic-ZC3H12D_PromoterWT

   Plasmid

   #153065

   Purpose

   ZC3H12D promoter region cloned upstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   ZC3H12D promoter region (ZC3H12D Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   None

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK_lacO-cP-AOX1-AtX+NpgA

   Plasmid

   #126727

   Purpose

   yeast plasmid overexpressing AtX and NpgA

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   atX,npgA
   

   Tags

   6xHis

   Expression

   Yeast

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TO-EGFP-RIP2_3K

   Plasmid

   #131205

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

   Insert

   RIPK2 K209/410/538R (RIPK2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K209/410/538R

   Promoter

   pCMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

    Plasmid

    #127309

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127317

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKdH_P-GAP-ScADH2

    Plasmid

    #126734

    Purpose

    yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK_-P-GAP-NpgA+AtX

    Plasmid

    #126725

    Purpose

    yeast plasmid overexpressing AtX and NpgA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    atX,npgA
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKdH_P-GAP-ScADH2+P-GAP*-ScALD6

    Plasmid

    #126737

    Purpose

    yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    adh2,ald6
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZ_P-ICL1-LacI-Mit1AD

    Plasmid

    #126721

    Purpose

    LacI-Mit1AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Mit1 activation domain coding sequence
    

    Expression

    Yeast

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZ_P-ICL1-LacI-VP16

    Plasmid

    #126723

    Purpose

    LacI-VP16 on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-VP16 activation domain coding sequence
    

    Expression

    Yeast

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZ_P-ICL1-LacI-Gal4AD

    Plasmid

    #126724

    Purpose

    LacI-Gal4AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Gal4 activation domain coding sequence
    

    Expression

    Yeast

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZ_lacO-cP-AOX1-sLovA

    Plasmid

    #126741

    Purpose

    yeast plasmid overexpressing sLovA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii
    

    Tags

    6xHis

    Expression

    Yeast

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127325

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127320

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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