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pCAG-SRGAP2A-EGFP

Plasmid

#168501

Purpose

Expression of full length SRGAP2 with a C-termonal EGFP fusion

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A (SRGAP2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163057

Purpose

Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-ORF9b

Plasmid

#165122

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF9b

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3TAG8li-hRNF160-3FLAG

Plasmid

#159138

Purpose

human RNF160 (LTN1, ZNF294) FLAG mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

listerin E3 ubiquitin protein ligase 1 (LTN1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake1

Plasmid

#139340

Purpose

Expresses Curlcake1 (CC1) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake1 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake2

Plasmid

#139341

Purpose

Expresses Curlcake2 (CC2) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake2 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake4

Plasmid

#139343

Purpose

Expresses Curlcake4 (CC4) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake4 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_FL

Plasmid

#157867

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-EGFP-Donor

Plasmid

#159740

Purpose

Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

EGFP

Use

Intron tagging donor

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

Plasmid

#159899

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmIrf1

Plasmid

#160098

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2

Plasmid

#159603

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

NIR-GECO2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDNA2.0-6H-TEV-KRAS G12D

Plasmid

#159439

Purpose

Expresses KRAS G12D in E.coli

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS G12D (KRAS Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Tags

FLAG-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Human SLC SAM Library

Pooled Library

#132561

Purpose

Activation library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Article

Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only
pCMV-BE4max-NRRH

Plasmid

#136918

Purpose

Expresses BE4max-NRRH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRRH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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