We narrowed to 23,642 results for: ESC
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Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHmK-C1
Plasmid#87866PurposeBackbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1DepositorInsertpHluorin-mKate2 fusion
TagspHluorin-mKate2 fusionExpressionMammalianAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM27
Plasmid#133641PurposeIntegration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miRFP680-P2A-GFP
Plasmid#197164PurposeExpresses the protein of miRFP680-P2A-GFP in mammalian cellsDepositorInsertmiRFP680-P2A-GFP
UseAAVAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR2-CR
Plasmid#40255DepositorInsertAKAR2
TagsHis6 and XpressExpressionMammalianPromoterCMV IEAvailable SinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACT5FVMF(f)
Plasmid#110549PurposeControl plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008DepositorInsertEGFP, FV LTRs
UseQpcr control for titering foamy virusPromoterpromoter: MLV LTRAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-StagRFP-AktAR
Plasmid#173020PurposeYellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.DepositorInsertVenus-StagRFP-AktAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-itis pSel
Plasmid#195344PurposeSelection plasmid expressing non-fluorescent EGFP A111VDepositorInsertEGFP
UseCRISPRMutationA111VPromoterJ23119Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-I-N-CAAX
Plasmid#41686PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activationDepositorInsertDronpa145N-IntersectinDH-Dronpa145N-CAAX
TagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable SinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PEP108E
Plasmid#97394Purposebinary construct of plasma membrane-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
TagsPlasma membrane targetExpressionPlantAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
ExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EB3-3xmScarletI-N1
Plasmid#112928PurposeIn vivo visualization of microtuble plus ends (can be used for colocalization studies)DepositorInsertEB3
Tags3x mScarlet-IExpressionMammalianPromoterCMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-EGFP
Plasmid#65255Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertIi-Streptavidin and truncated Mannosidase II
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKEK3230
Plasmid#234102PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1
Plasmid#61625PurposeFRET-based biosensor for monitoring JNK activityDepositorInsertJNKAR1
TagsCitrine and ECFPExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dendra2-Golgi-7
Plasmid#57722PurposeLocalization: Golgi, Excitation: 490 / 553, Emission: 507 / 573DepositorInsertGolgi (B4GALT1 Human)
TagsDendra2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046768
Plasmid#145763PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits