We narrowed to 24,301 results for: FUS
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Plasmid#121408PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L313D I314K)
Plasmid#121390PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to L…PromoterADH1 promoterAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (L105D)
Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (F317D)
Plasmid#121406PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (I325D)
Plasmid#121407PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+C3(R112C/T180C)X
Plasmid#108196PurposeExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coliDepositorInsertP450cam-PCNA3(R112C/T180C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMOD_B3200
Plasmid#91079PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VPR_1)Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3400
Plasmid#91080PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VP64_1)Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
TagsYFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGD-I27-CC
Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CFa
Plasmid#85089PurposeExpresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coliDepositorInsertThe G108C variant of PCNA1 fused to an FAD domain of P450 BM3
TagsHis6 tagExpressionBacterialMutationG108C in PCNA1, A74G/C773S/C810S in FAD domain of…PromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLexA::SRDX (GB0732)
Plasmid#68184PurposeProvides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLexA Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD::BDR (GB0734)
Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBF-E6
Plasmid#21696DepositorInsertprotein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
TagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
ExpressionMammalianAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only