Addgene

We narrowed to 5,038 results for: Apoe

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptrc_BsMdh_BmHps_BmPhi

Plasmid

#129104

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ265L2

Plasmid

#174000

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-EE

Plasmid

#154147

Purpose

Expresses dLbCpf1-BE-EE(dLbCas12a-BE-EE) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-EE (dCas12a-BE-EE)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(R126E+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-RNF114

Plasmid

#58295

Purpose

mammalian expression of myc tagged RNF114

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE2 Lnp-mCherry

Plasmid

#86687

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

LNPK (LNPK Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCCM’

Plasmid

#162709

Purpose

pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Use

Tags

Expression

Bacterial

Mutation

p15A origin replaced with high-copy colE1 origin

Promoter

PLtet0-1 promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl1

Plasmid

#86678

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMW96

Plasmid

#178066

Purpose

Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

Depositor

Tarun Kapoor

Article

Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

Insert

CDK5RAP2 (CDK5RAP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Atl1

Plasmid

#86679

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-eGFP-dSaCas9-blast

Plasmid

#191653

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

eGFP-T2A-dSaCas9

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-dSaCas9-KRAB-blast

Plasmid

#191654

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

dSaCas9-KRAB

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1E10A

Plasmid

#84607

Purpose

Plasmid contains HCF-1rep1E10A with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1E10A (HCFC1 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1wt

Plasmid

#84606

Purpose

Plasmid contains HCF-1rep1wt with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1wt (HCFC1 Human)

Use

Tags

GST-TEV and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V-IRES-ASK1

Plasmid

#221074

Purpose

Expresses NRAS G12V and ASK1 in mammalian cells

Depositor

Hidenori Ichijo

Article

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Inserts

NRAS (NRAS Human)
ASK1

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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