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Showing: 881 - 900 of 1425 results

  3. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

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  5. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid
    #221843
    Purpose
    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Insert
    EGFP
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221843-plasmid-map-sequence-id-437471

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  6. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  9. pMH184

    Plasmid
    #122857
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122857-plasmid-map-sequence-id-239400

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  10. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid
    #221845
    Purpose
    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221845-plasmid-map-sequence-id-437474

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  11. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid
    #221846
    Purpose
    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote
    Depositor
    Stephen Terry
    Article
    O’Sullivan et al bioRxiv 2024.03.04.583298
    Inserts
    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGC and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    221846-plasmid-map-sequence-id-437473

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  12. CMV:AsCpf1-2A-GFP-U6-INS-sg

    Plasmid
    #194719
    Purpose
    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS
    Depositor
    Rudolf Jaenisch
    Article
    Haiting et al bioRxiv
    Insert
    AsCpf1 (INS Acidaminococcus sp.)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    194719-plasmid-map-sequence-id-381224

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  15. KJ740(DE3)

    Bacterial Strain
    #179486
    Purpose
    KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Bacterial Resistance
    Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)
    Species
    Escherichia coli
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166133
    Purpose
    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Raphael Gaudin
    Article
    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep
    Insert
    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166133-plasmid-map-sequence-id-333282

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Showing: 881 - 900 of 1425 results