We narrowed to 90,284 results for: Mal;

1. pRK5-HA-Sestrin2

   Plasmid

   #72593

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. CROPseq-Guide-BSD

   Plasmid

   #216123

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-BHMT-I/G

   Plasmid

   #104442

   Purpose

   Autophagy cargo assay

   Depositor

   Carol Mercer

   Article

   Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

   Insert

   BHMT (BHMT Human)
   

   Tags

   GFP-myc GFP is from Aequorea victoria and GST

   Expression

   Mammalian

   Mutation

   N43A in GST (in a potential KFERQ-like sequence).…

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hST3GAL6-RFP

   Plasmid

   #22923

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-5X-1-Full-Length-MePCE

   Plasmid

   #113539

   Purpose

   Produces an N-terminal GST-tagged full length MePCE protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

   Plasmid

   #113889

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

   Plasmid

   #40809

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S218E, S222D

   Promoter

   CMV

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pABCb10-GFP

   Plasmid

   #19031

   Depositor

   Orian Shirihai

   Article

   Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

   Insert

   ATP-binding cassette, sub-family B (MDR/TAP), member 10 (Abcb10 Mouse)
   

   Tags

   HIS

   Expression

   Mammalian

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM-AAV-mSncg-EGFP

    Plasmid

    #153163

    Purpose

    Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mSncg

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-TSF-TEVcs-ULK1K46I

    Plasmid

    #171417

    Purpose

    For the purifcation of human ULK1 kinase dead complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    kinase dead

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK312 (TOP2A CRISPR)

    Plasmid

    #140654

    Purpose

    TOP2A tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hNPPC-RFP

    Plasmid

    #22921

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP-1 mu1-GFP

    Plasmid

    #173432

    Purpose

    Expression of AP-1 Mu1 subunit with a terminal GFP

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP1M1 (Ap1m1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Target-AIDmax

    Plasmid

    #139107

    Purpose

    Mammalian Target-AIDmax expression vector (pSI-626)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. SMC2 CRISPR

    Plasmid

    #140649

    Purpose

    SMC2 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    SMC2 (SMC2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

    Plasmid

    #133282

    Purpose

    Lentiviral expression of a peroxisomal eGFP

    Depositor

    Etienne Sokal

    Article

    Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

    Insert

    Peroxisome targeting signal 1
    

    Use

    Lentiviral

    Tags

    Peroxisome targeting signal 1

    Expression

    Mammalian

    Promoter

    None

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid

    #155227

    Purpose

    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp in original Ezrin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 12x PRE TK luc

    Plasmid

    #206163

    Purpose

    Luciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    12X PRE TK luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    min TK

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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