We narrowed to 62,357 results for: cloning
-
Plasmid#83180PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutationDepositorAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
-
Hs.NRAS Q61R
Plasmid#83179PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutationDepositorAvailable SinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E347 Hs.TIAM1
Plasmid#70631PurposeGateway ORF clone of human TIAM1 [NM_003253.2] with stop codon (for native or N-terminal fusions)DepositorInsertTIAM1 (TIAM1 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E318 Hs.SOS1-nostop
Plasmid#70602PurposeGateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)DepositorInsertSOS1 (SOS1 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E229 Hs.RASA2
Plasmid#70513PurposeGateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)DepositorInsertRASA2 (RASA2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E084 Hs.FGFR1-nostop
Plasmid#70368PurposeGateway ORF clone of human FGFR1 [NM_001174063.1] without stop codon (for C-terminal fusions)DepositorInsertFGFR1 (FGFR1 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E085 Hs.FGFR2
Plasmid#70369PurposeGateway ORF clone of human FGFR2 [NM_000141.4] with stop codon (for native or N-terminal fusions)DepositorInsertFGFR2 (FGFR2 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-gRNA2.1
Plasmid#170512PurposegRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-MCS-P2A-GFP-PuroR
Plasmid#107186PurposeBase plasmid for cloning gene duplication alleles with eGFP reporter.DepositorTypeEmpty backboneAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB413
Plasmid#74807PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus
Plasmid#214678PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
B52 + FANCM sgSTOP
Plasmid#100710PurposeB52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + CHEK2 sgSTOP
Plasmid#100712PurposeB52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A
Plasmid#122155Purposerat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)DepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
ExpressionBacterialAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Zeo
Plasmid#227268PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME mKate2-dynactin1-811
Plasmid#105971Purposeentry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811DepositorInsertdynactin1-811 (DCTN1 Human)
TagsmKate2ExpressionBacterialMutationcontains only amino acids 1-811Available SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only