Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,247 results for: Arch

Showing: 9021 - 9040 of 9247 results

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-7a)

Plasmid

#241068

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-7a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-7a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,S155R,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4a)

Plasmid

#241065

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,P25del,P26del,A27S,T76I,G142D,M153S,V213G,G3…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4b)

Plasmid

#241066

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,T76I,G142D,M153S,V…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2g)

Plasmid

#241064

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2g) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2g) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S155R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2e)

Plasmid

#241062

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2e) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2e) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K529L,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2f)

Plasmid

#241063

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2f) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2f) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K147N,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2c)

Plasmid

#241060

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2d)

Plasmid

#241061

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

F490R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2a)

Plasmid

#241058

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2h)

Plasmid

#241059

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2h) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2h) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2)

Plasmid

#241056

Purpose

Expresses SARS-CoV-2 spike (BA.2) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1)

Plasmid

#241057

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD_1-270

Plasmid

#218229

Purpose

Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 1-270

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_1-275

Plasmid

#218230

Purpose

Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 1-275

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_29-484

Plasmid

#218231

Purpose

Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 29-484

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_29-270

Plasmid

#218232

Purpose

Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 29-270

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLCKO-CMV-ACE2-P2A-Blasticidin

Plasmid

#237476

Purpose

Encodes full length human ACE2 protein, along with blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A-Blasticidin

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX_307-SARS-CoV-2-NSP3-V5

Plasmid

#214429

Purpose

Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SARS-CoV-2 NSP3 (part of SARS-CoV-2 ORF1ab) (ORF1ab )

Use

Lentiviral

Tags

V5 tag

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K197R-VA

Plasmid

#191223

Purpose

Lentiviral expression of human IFIT5_K197R in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 197 to Arginine (K197R)

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

Plasmid

#191217

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Alpha (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 9021 - 9040 of 9247 results

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