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NAX_CAGGS_CAS9_m4_Puro

Plasmid

#167871

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB311

Plasmid

#68639

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB132

Plasmid

#68586

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-Casp2

Plasmid

#50023

Purpose

pENTR vector for mouse Caspase-2

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Casp2 (Casp2 Mouse)

Use

Entry vector

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFvL118

Plasmid

#64752

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB124

Plasmid

#68578

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMpGWB100

Plasmid

#68062

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PGL3-MK17

Plasmid

#21486

Depositor

Shoumo Bhattacharya

Article

Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

Insert

Cited2 Promoter (CITED2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFIN-XOPS-tdTOM

Plasmid

#44359

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

XOPS-tdTOM

Use

Lentiviral

Promoter

Xenopus Opsin (XOPS) (pos 3125-4501)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB328

Plasmid

#68656

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBSc + MShab

Plasmid

#16146

Depositor

Larry Salkoff

Article

Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

Insert

Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pUM31dao

Plasmid

#87242

Purpose

Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

Depositor

Anil Day

Article

Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

Insert

D-amino oxidase

Expression

Plant

Promoter

plastid rrn

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Tags

mMaple3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPbeta

Plasmid

#13393

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPbeta (Cebpb Rat)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only