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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,447 results for: grn

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Showing: 9081 - 9100 of 15447 results

  2. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126762-plasmid-map-sequence-id-410965

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  4. B52 + SPRTN sgSTOP

    Plasmid
    #100709
    Purpose
    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting SPRTN (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100709-plasmid-map-sequence-id-193207

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  5. pEE495

    Plasmid
    #149291
    Purpose
    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Publication
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Expression
    Plant
    Promoter
    35S
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149291-plasmid-map-sequence-id-305898

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  8. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Publication
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  9. pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

    Plasmid
    #101285
    Purpose
    Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP
    Depositor
    Alpha Yap
    Publication
    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do
    Insert
    E-Cadherin-GFP-H-Ras
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    H-Ras V12
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101285-plasmid-map-sequence-id-197226

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  13. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Publication
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TK2 (TK2 Human)
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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  14. pUC-H1-Esp3I-mU6-Kan

    Plasmid
    #213015
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)
    Depositor
    Stephanie Roessler
    Insert
    H1 promoter, mU6 promoter, gRNA scaffolds
    Promoter
    H1 promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213015-plasmid-map-sequence-id-423154

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  15. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  16. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  18. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  19. pDual_dsCas9_Puro

    Plasmid
    #214680
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214680-plasmid-map-sequence-id-428814

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  20. pSilencer-Opa1 KO

    Plasmid
    #191949
    Purpose
    Expression of a siRNA that targets Opa1
    Depositor
    Luca Scorrano
    Insert
    Opa1
    Expression
    Mammalian
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    191949-plasmid-map-sequence-id-379580

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Showing: 9081 - 9100 of 15447 results